Nonexponential Fluorescence Decay of Tryptophan, Tryptophylglycine, and Glycyltryptophan

Metal-peptide Interaction: Influence of the Aminoacid Sequence on the Binding of Co(II) to Glycyltryptophan and Tryptophylglycine Studied by ^NMR and Fluorescence

Nonexponential Fluorescence Decay in Tryptophan and Tryptophan-containing Peptides and Proteins

The major difficulty in using tryptophan fluorescence decay to probe the structure and dynamics of peptides and proteins is the intrinsic nonexponential decay of tryptophan itself. We have recently proposed a model which rationalizes the nonexponential deca¥ of Trp in terms of conformers about the Ca_CB, or x , bond. See figure 1. The underlying assumption of this

Ionization potentials of fluoroindoles and the origin of nonexponential tryptophan fluorescence decay in proteins.

This work reports an explanation for the unusual monoexponential fluorescence decay of 5-fluorotryptophan (5FTrp) in single-Trp mutant proteins [Broos, J.; Maddalena, F.; Hesp, B. H. J. Am. Chem. Soc. 2004, 126, 22-23] and substantially clarifies the origin of the ubiquitous nonexponential fluorescence decay of tryptophan in proteins. Our results strongly suggest that the extent of nonexponenti...

Nonexponential fluorescence decay of aqueous tryptophan and two related peptides by picosecond spectroscopy.

Time-resolved fluorescence spectroscopy of tryptophan and two related dipeptides, tryptophylalanine and alanyltryptophan, has been carried out on the subnanosecond time scale by using picosecond exciting pulses at a wavelength of 264 nm. Detection was with an ultrafast streak camera coupled to an optical multichannel analyzer. The zwitterions of these molecules show a definite nonexponential fl...

Photophysics of Aqueous Tryptophan: pH and Temperature Effects

The fluorescence decay kinetics of aqueous tryptophan and 3-methylindole have been determined as a function of pH and temperature by using a picosecond dye laser-single photon counting system with a time resolution of 50 ps. At pH 11, tryptophan exhibits a single exponential decay, with a lifetime of 9.1 ns at 18 O C . However, at pH 7 the decay is faster and definitely nonexponential; the valu...